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Import Quarantine Material
In some cases, "Permit to Import Quarantine Material" is required for shipment of biological materials (Fig.1.4).
For US transfer, please refer to the well-documented website at
http://www.ehs.umaryland.edu/osh/importation.htm or search for an appropriate page using Google Search.
The following Table shows current information relevant to adenoviruses.
| Type of adenovirus |
Human adenovirus type 5 |
| Treatment of material PRIOR to importation into your country (processing/purification methods, including time at specific temperatures, pH, other treatment, disease safeguard, etc.) |
The cells infected with recombinant adenovirus were sonicated and frozen at -80C. |
| Cell line or reference number (for the cells used to produce the adenovirus) |
HEK293 obtained from ATCC. |
| Cell line passage history |
Within 20 passages after obtaining from ATCC. The cells were cultured in Japan and dispatched from Japan. |
| Type of culture medium (basic or special) |
DMEM |
| Identify nutritive factors in medium (protein/nitrogen sources used and/or added: specify country of origin, in particular in the case of supplements or nitrogen sources of animal origin) |
Supplemented with 5% fetal bovine serum (FBS) that was inactivated by incubation at 56oC for 45 min.The FBS (Invitrogen/Life Technologies) was imported from Australia to Japan. This FBS is derived from animals that received ante and post mortem inspection at the time of slaughter and were found free of infectious diseases. Please ask a data sheet to us (dnabank@brc.riken.jp). |
| Country of origin and sources of enzymes used (e.g. trypsin) |
Trypsin, Canada (Invitrogen) |
| Names of any animal viruses studied in the lab in which the cell line originated |
The cells were not exposed to infectious agents of agricultural importance. |
| If recombinant, specify genetic insert |
Refer to the web catalog for specific information about each recombinant virus. You can obtain information by searching by RDB no. |
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RIKEN DNA Bank documents (forms) are now available in PDF format via the worldwide web. These documents are printable and can be viewed using Adobe Reader, which comes with many web browsers and is available free of charge from Adobe's website at http://www.adobe.com.
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Order forms
Researchers who want to obtain RDB recombinant adenoviruses, please read "Import Quarantine Material"
| Forms |
Description |
| Form A |
Order form
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| Form C |
Material Transfer Agreement
We ask a signature of the Authorized Representative by an administrator of intellectual property.
(Please e-mail to dnabank@brc.riken.jp to obtain MTA in MS-Word format) |
| Form D |
Approval form
In the case Depositor and/or Developer require written consent. |
| Form E |
Agreement of secondary supply for CAG promoter
In the case a clone contains CAG promoter. |
Obtain FormA.
| Forms |
Description |
PDF |
| Form A |
Order Form |
pdf_receive.pdf |
Find and obtain appropriate FormC (Material Transfer Agreement).
(Please e-mail to dnabank@brc.riken.jp to obtain MTA in MS-Word format)
Please use an appropriate category of Material Transfer Agreement (MTA).
- Category I MTA
- For not-for-profit academic researches by not-for-profit institutions.
- Category II MTA
- Applicable for the following use:
- By for-profit institutions.
- For collaborative researches by not-for-profit institutions with for-profit institutions.
- For contract researches by not-for-profit institutions financed by for-profit institutions.
- For researches by not-for-profit institutions for financial gain, including researches that directly aim to obtain patent.
In addition to Category I or II MTA, certain resources require prior written permission of the depositors.
Additional forms required
Some of the DNA clones are restricted on use and require the written consent of the depositor or developer.
| Forms |
Description |
PDF |
Word |
| Form D |
Approval form
In the case Depositor and/or Developer require written consent.
| | Clones are provided |
| b. | with written consent of depositor |
| c. | with written consent of developer |
| d. | with written consent of depositor and developer |
|
05FormD.pdf |
05FormD.doc |
| Form E |
Agreement of secondary supply for CAG promoter
If you want clones with the CAG promoter (indicated by "g" in the catalog), the "Agreement of secondary supply for the CAG promoter (Form E)" is also required. |
(04Form_E-1.pdf)
(for a Not-For-Profit Organization)
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Regarding "Approval form (03Form D)", please complete it and send it to the depositor or developer for permission (Fig.1.2). Then, please return the completed form together with other forms to us (Fig.1.3). Addresses of depositors and developers are listed in the "Gene Catalog" of the RIKEN DNA Bank.
If you cannot find a name and address in the catalog, or if you have any questions, please contact us by e-mail or by FAX.
Form E-1 is processed by us and you need not send this form to Dr. Miyazaki (Osaka Univ. Medical School; Fig.1.5).
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Address for Orders
Complete an "Order Form (03Form A)" for each order.
Make two copies of the "Material Transfer Agreement (MTA; 03Form C)" for each material.
Send forms A, C, D and E-1 to RIKEN DNA Bank (Fig.1.3):
The DNA Bank, RIKEN Tsukuba Institute,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
- If you are not sure that you have completed forms correctly, please send them to us by e-mail or fax for us to look them over.
- Please inform us an ID of MTA and PO of your institute by e-mail, if applicable.
To contact us:
- Address: The DNA Bank, RIKEN Tsukuba Institute,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074 Japan
- Fax: (+81)-29-836-9120
- E-mail:
dnabank@brc.riken.jp
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Our distribution procedures
- The MTA is sent with materials (Fig.1.5).
- All DNA clones requested from abroad will be sent to you by air-mail. We recommend picking at least 5 independent colonies for verification to ensure that the clone of interest is derived from a single isolate.
- We usually send recombinant adenoviruses at room temperature.
Please refer to our website (http://www.brc.riken.jp/lab/dna/en/adenovirus.html) for details about the stability of recombinant adenoviruses.
For transport of recombinant adenoviruses with dry ice, additional shipping fees are required. If you wish for frozen transport, please indicate your request on Form A.
- The invoice (bill) will be sent to you separately (Fig.1.7) after you have received the requested biological materials (Fig.1.6).
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Payment and charges
3.1 Price
See Table at
http://www.brc.riken.jp/inf/en/news/20100312.html
The revised distribution fees are applied to all orders for the bioresources after 08:00 GMT on March 19, 2010
Terms and conditions of sale
- Above prices do not include shipping charges. Additional fees will be charged for shipping.
- We usually send recombinant adenoviruses at room temperature. Please refer to our website (http://www.brc.riken.jp/lab/dna/en/adenovirus.html) for details about the stability of recombinant adenoviruses. For transport of recombinant adenoviruses with dry ice, additional shipping fees are required (Fig.1.7). If you choose frozen transport, please indicate your choice on Form A.
- Products and prices are correct at the time of printing but are subject to change without notice.
- The following are included in 'Plasmid, Cosmid and Host Strain':
Cloned DNA, vector and bacteria
CEPH MEGA YAC clone
MSM Mouse BAC clone
NIA/NIH Mouse cDNA clone
ERATO preimplantation mouse embryonic cDNA clone
HLA cDNA expression vector
SEREX cDNA clone
3.2 Payment
An INVOICE will be sent you after you have received the requested materials (Fig.1.7).
3.2.1 International users
Please pay in either of the following two ways (after you have received your INVOICE):
3.2.1.1 Remit the charge to the following account.
3.2.1.2 Send a bank check, in Japanese yen.
Details are indicated at:
http://www.brc.riken.jp/inf/en/distribute/pay.shtml
3.2.2 Domestic users
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Quality control
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Individual clone
- Each DNA sample in our Bank is examined by gel-electrophoretic analysis of digested DNA. In some cases, we verify the nature of DNA samples by nucleotide sequencing. After obtaining clones, users are recommended picking at least 5 independent colonies for verification to ensure that the clone of interest is derived from a single isolate and verifying the clones with restriction digestion by enzyme and/or nucletide sequencing.
Clone details can be viewed within our on-line catalog. When browsing search results, the browser window provides access to additional information such as vector maps, antibiotic resistance, sequences provided by the depositor, and much more.
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Clone set
- Plate-row-column coordinates, DNA sequences, and annotations originate from the depositor and have not been independently verified by RIKEN DNA Bank. We do, however, curate and act upon user reported problems with clones.
We therefore strongly recommend that users picking at least 5 independent colonies for verification to ensure that the clone of interest is derived from a single isolate, and that user should verify the clones with restriction digestion by enzyme and/or nucletide sequencing.
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Follow-up survey
To obtain information about the quality of the genetic resources that we have sent, we request all recipients to complete a "follow-up" survey. Please fill out and return the follow-up questionnaire enclosed in the package. If you have any questions about the "quality" of the materials, please contact us by FAX or e-mail immediately.
Fax: (+81)-29-836-9120; E-mail:
dnabank@brc.riken.jp
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Request for publications
We wish to share all data about the genetic resources in the RIKEN DNA Bank. Therefore, please send us reprints after the publication of any reports of experiments with our materials.
We have been asked by MEXT to ensure that all investigators include a acknowledgement in publications that benefit from the use of the RIKEN BRC's resource. We suggest that the following statement be used:
________ (name of BIOLOGICAL RESOURCE) was provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan.
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