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RIKEN BRC

Technology and Development Team for Mammalian Cellular Dynamics
introduction

   We develop technologies to monitor and to analyze dynamic nature of mammalian cells, which will be useful tools for phenotyping biological resources collected at the BioResource Center.
   In order to achieve this goal, our team uses genetic, genomic and transgenic/gene targeting approaches.

 Team Leader Head Kuniya ABE, PH.D.
Team Leader
Head
Kuniya ABE, PH.D.

Development of Technology
Fig.1
  • Visualization of pluripotent embryonic stem cells and germ cells, and establishment of methods and resources for the analyses of genomic reprogramming processes.
  • Monitoring changes in nuclear organizations and epigenetic status during stem cell differentiation.
  • Novel research tools to analyze expression and functions of non-coding RNAs during development.
  • Development of resources derived from wild mouse strains collected at BRC.

Projects

Development of methods for

  1. Analyses of molecular mechanisms controlling proliferation and differentiation of embryonic stem cells/ germ cells.
  2. Comprehensive studies on gene expression in embryonic stem cells/primordial germ cells
  3. Monitoring dynamics of nuclear reorganizations during embryonic development.
  4. Functional genomic analyses using BAC genomic clones and genetic polymorphism (SNPs) derived from Asian wild mouse strains.
 
Fig.1 Fig.2
Developmental analysis of mutation affecting embryonic ectoderm development
(Sugimoto et al., Genesis 37, 162-171, 2003)

 
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